22 posts · 24,117 views

Sort by Latest Post, Most Popular

View by Condensed, Full

  • March 28, 2011
  • 11:26 AM

A call for increased use of pharamacogenomic data in the clinic

by avi_wener in American Biotechnologist

In a recent review article published in the New England Journal of Medicine, authors Liewei Wang and a team from the Mayo Clinic discuss recent advances in the field of pharmacogenomics and the potential value that it holds for patient care with proper uptake by the medical community. Pharmacogenomics is the study of a patient’s [...]... Read more »

Wang L, McLeod HL, & Weinshilboum RM. (2011) Genomics and drug response. The New England journal of medicine, 364(12), 1144-53. PMID: 21428770  

  • January 20, 2011
  • 03:52 AM

Real-Time Visualization of DNA Transcription

by avi_wener in American Biotechnologist

Most of my graduate work focused on transcriptional regulation of a vasoregulatory gene and all the nitty-gritty work that goes along with these types of molecular protocols. As such, I am always on the lookout for techniques that improve upon current transcriptional regulation protocols especially if they show a propensity for doing the job either [...]... Read more »

L. Stirling Churchman, & Jonathan S. Weissman. (2011) Nascent transcript sequencing visualizes transcription at nucleotide resolution. Nature, 368-373. info:/doi:10.1038/nature09652

  • January 17, 2011
  • 07:11 AM

Arcade Games Biotic Style

by avi_wener in American Biotechnologist

We found a great post this past weekend on fastcompany.com which we thought we’d share with you. Author David Zax describes an interactive video game designed by researchers in the bioengineering department of Stanford University that uses living cells as part of the game mechanics. These “Biotic Games” are played much like any arcade classic [...]... Read more »

Riedel-Kruse IH, Chung AM, Dura B, Hamilton AL, & Lee BC. (2011) Design, engineering and utility of biotic games. Lab on a chip, 11(1), 14-22. PMID: 21085736  

  • December 7, 2010
  • 02:31 PM

Scientific Discovery of the Year or Grandiose Hyperbole?

by avi_wener in American Biotechnologist

Towards the end of last week, NASA announced that it would reveal a stunning scientific report in a live press conference that would surely be the scientific breakthrough of the year. Watching the conference later in the day, I was amazed to hear Felisa Wolfe-Simon of the NASA Astrobiology Institute explain that she found a [...]... Read more »

Wolfe-Simon F, Blum JS, Kulp TR, Gordon GW, Hoeft SE, Pett-Ridge J, Stolz JF, Webb SM, Weber PK, Davies PC.... (2010) A Bacterium That Can Grow by Using Arsenic Instead of Phosphorus. Science (New York, N.Y.). PMID: 21127214  

  • November 24, 2010
  • 01:30 PM

Giving Thanks for Turkey Science

by avi_wener in American Biotechnologist

What does the domesticated Turkey have in common with the mouse, rat, chimpanzee and Human? Give up? The answer is that its genome can now be added to the long list of species that have had their genomes sequenced thanks to advances in Next Generation Sequencing.
Earlier this year, a team of 34 scientists published their [...]... Read more »

  • October 20, 2010
  • 11:45 AM

Genomic Evolution in the Blink of an Eye

by avi_wener in American Biotechnologist

If you think that genetic evolutionary processes take Milena….think again. Scientists at Allegheny General Hospital’s Center for Genomic Sciences have found that through a process known as horizontal gene transfer (HGT), bacterial pathogens can mutate multiple times during the course of a bacterial infection. In a study published by Hiller et. al. in PLoS Pathogens [...]... Read more »

  • July 29, 2010
  • 11:40 AM

qPCR Analysis: It’s What’s Inside That Counts

by avi_wener in American Biotechnologist

If you watched the video on real time quantitative PCR data analysis, you should have a good understanding of real-time quantitative PCR basics and the associated data analysis techniques. Classical quantification techniques such as Livak, delta CT and the Pfaffl rely on linear regression analysis and are currently the most widely accepted methodologies for quantitative [...]... Read more »

  • July 26, 2010
  • 07:30 AM

Sustainable RNA Transfection

by avi_wener in American Biotechnologist

MIT scientists Matthew Angel and Mehmet Fatih Yanik have discovered a method for transfecting mRNA into fibroblasts without triggering the immune response that normally defends cells against exogenous RNA infection. Cells are usually able to differentiate between endogenous and exogenous RNA through activation of pattern-recognition receptors (PRRs) that initiate a subsequent immune response. While this [...]... Read more »

Angel M, & Yanik MF. (2010) Innate Immune Suppression Enables Frequent Transfection with RNA Encoding Reprogramming Proteins. PLoS ONE, 5(7). info:/

  • July 15, 2010
  • 09:49 AM

More Fecal Findings!

by avi_wener in American Biotechnologist

At the risk of developing a complex that all I talk about is fecal matter, for the second time this week I would like to bring your attention to another study that focuses on the gut and its microbial habitat. A couple of days ago I discussed the challenge of identifying the huge number of [...]... Read more »

Alejandro Reyes, Matthew Haynes, Nicole Hanson, Florent E. Angly, Andrew C. Heath, Forest Rohwer, & Jeffrey I. Gordon. (2010) Viruses in the faecal microbiota of monozygotic twins and their mothers. Nature, 334-338. info:/10.1038/nature09199

  • June 29, 2010
  • 12:21 PM

Cultured Stem Cells Used to Restore Vision Loss Caused by Corneal Burns

by avi_wener in American Biotechnologist

A recent study published in the New England Journal of Medicine reported that autologous limbal stem cells can be used to permanently reverse the loss of vision caused by ocular burn induced limbal stem-cell deficiency (limbal stem cells function to replace dead corneal cells).
It is important to note that in the cases reported, eyesight [...]... Read more »

Rama, P., Matuska, S., Paganoni, G., Spinelli, A., De Luca, M., & Pellegrini, G. (2010) Limbal Stem-Cell Therapy and Long-Term Corneal Regeneration. New England Journal of Medicine. DOI: 10.1056/NEJMoa0905955  

  • June 23, 2010
  • 07:10 AM

Interdisciplinary Approach Creates New Tools for Protein Scientists

by avi_wener in American Biotechnologist

One of my favorite pastimes is reading about situations where experts from different (and seemingly divergent) disciplines get together to discuss interdisciplinary cooperation and mutual progress. All too often scientists segregate themselves according to their area of expertise and only make use of the techniques that they are familiar with or have access to in [...]... Read more »

Finney, L., Chishti, Y., Khare, T., Giometti, C., Levina, A., Lay, P., & Vogt, S. (2010) Imaging Metals in Proteins by Combining Electrophoresis with Rapid X-ray Fluorescence Mapping. ACS Chemical Biology, 5(6), 577-587. DOI: 10.1021/cb1000263  

  • June 21, 2010
  • 04:36 AM

Linking Diabetes and Cancer: Where’s the Evidence?

by avi_wener in American Biotechnologist

If you’ve been following the Diabetes news lately, you likely noticed that two stories have dominated the news over the last couple of days. The first is a widely published study that implicates the consumption of white rice (as opposed to brown rice) in increased incidents of diabetes and the second relates to emerging evidence [...]... Read more »

Giovannucci, E., Harlan, D., Archer, M., Bergenstal, R., Gapstur, S., Habel, L., Pollak, M., Regensteiner, J., & Yee, D. (2010) Diabetes and Cancer: A Consensus Report. CA: A Cancer Journal for Clinicians. DOI: 10.3322/caac.20078  

  • June 2, 2010
  • 11:41 AM

Biological Bullets for Wayward Cells

by avi_wener in American Biotechnologist

Transfecting cells can be a difficult task for even the most seasoned of cell biologists. Sure there are easy-to-transfect cell lines such as A10 Smooth Muscle Cells, COS Cells or p19 cells, but what about those pesky, hard to isolate primary cells? Or better yet, what about cells embedded in living tissue on animals or [...]... Read more »

  • May 31, 2010
  • 11:01 AM

Enteroviral Replication Revealed

by avi_wener in American Biotechnologist

Enteroviruses such as Poliovirus and Hepatitis are notorious for the disastrous effects they cause upon infection. A study recent published in Cell contributes a significant finding to the field of RNA virus replication. The Altan-Bonnet lab at Rutgers University has shown how that upon infection, RNA viruses generate specialized RNA replication organelles enriched in phosphatidylinositol-4-phosphate [...]... Read more »

Hsu, N., Ilnytska, O., Belov, G., Santiana, M., Chen, Y., Takvorian, P., Pau, C., van der Schaar, H., Kaushik-Basu, N., & Balla, T. (2010) Viral Reorganization of the Secretory Pathway Generates Distinct Organelles for RNA Replication. Cell, 141(5), 799-811. DOI: 10.1016/j.cell.2010.03.050  

  • May 25, 2010
  • 03:34 PM

Evolution of Darwin: Hollywood Style

by avi_wener in American Biotechnologist

According to popular thought, Charles Darwin’s Origin of the Species was heavily influenced by his grandfather Erasmus Darwin (1). The impact that a grandparent can have upon an impressionable child should never be underestimated. To what extent do you think that Dana Carvey was influenced by his grandfather?

Dana Carvey is “DARWIN” – watch more funny [...]... Read more »

  • May 25, 2010
  • 10:16 AM

Mapping the Human Brain

by avi_wener in American Biotechnologist

The Allen Institute for Brain Science initiated the Allen Brain Atlas in 2003 with a goal to create a genomic map of the mouse brain. The mouse brain atlas was successfully completed in 2006 using 85 million images containing 600 terabytes of data and identifying 21,000 active genes in the mouse brain. The atlas has [...]... Read more »

Lein, E., Hawrylycz, M., Ao, N., Ayres, M., Bensinger, A., Bernard, A., Boe, A., Boguski, M., Brockway, K., Byrnes, E.... (2006) Genome-wide atlas of gene expression in the adult mouse brain. Nature, 445(7124), 168-176. DOI: 10.1038/nature05453  

Jones AR, Overly CC, & Sunkin SM. (2009) The Allen Brain Atlas: 5 years and beyond. Nature reviews. Neuroscience, 10(11), 821-8. PMID: 19826436  

  • May 24, 2010
  • 07:45 AM

Look Mom…Molecular Biologists On TV

by avi_wener in American Biotechnologist

As scientists we are “naturally obsessed.” We have a need to find the answer and can’t stop working until we get it. So how do we find the time to watch this one hour dramatic story by Richard and Carole Rifkind about life in a molecular biology lab? Although there are many health related stories [...]... Read more »

  • May 21, 2010
  • 10:00 AM

Proteomics Refined

by avi_wener in American Biotechnologist

As a proteomics fan in general and a 2D Gel Electrophoresis fan specifically, I have been following the Ludesi Blog which I have found to be a resource rich in 2D information including a knowledgebase, webinars and an great tool that pulls together all proteomics related tweets from twitter. For those of you who [...]... Read more »

  • May 20, 2010
  • 04:13 PM

Transforming Species One Genome at a Time

by avi_wener in American Biotechnologist

Gibson et. al. from the Venter Institute have just published a report in Science Express (online only) that sounds very cool, but whose implications don’t seem to be well understood yet from experts in the field. Essentially the group synthesized the genome of one bacterial species and transplanted it into another bacterial species. What resulted [...]... Read more »

Lartigue, C., Vashee, S., Algire, M., Chuang, R., Benders, G., Ma, L., Noskov, V., Denisova, E., Gibson, D., Assad-Garcia, N.... (2009) Creating Bacterial Strains from Genomes That Have Been Cloned and Engineered in Yeast. Science, 325(5948), 1693-1696. DOI: 10.1126/science.1173759  

Lartigue, C., Glass, J., Alperovich, N., Pieper, R., Parmar, P., Hutchison, C., Smith, H., & Venter, J. (2007) Genome Transplantation in Bacteria: Changing One Species to Another. Science, 317(5838), 632-638. DOI: 10.1126/science.1144622  

  • May 17, 2010
  • 07:00 AM

Simplifying Gene Knockdown Experiments

by avi_wener in American Biotechnologist

If you’ve ever done gene knockdown experiments, you know that the process can be complicated and involve many steps. siRNA design, cell culture, perfect cell confluency, transfection or electroporation, downstream analysis etc. Bio-Rad now has a plethora of instruments and reagents to help you simplify the process. In a recently published article in the Journal [...]... Read more »

join us!

Do you write about peer-reviewed research in your blog? Use ResearchBlogging.org to make it easy for your readers — and others from around the world — to find your serious posts about academic research.

If you don't have a blog, you can still use our site to learn about fascinating developments in cutting-edge research from around the world.

Register Now

Research Blogging is powered by SRI Technology.

To learn more, visit http://selfregulationinstitute.org/.